KVV E-Modules - Culture & Sensitivity Test

Quadrant -I (Video)

Culture & Sensitivity Test

Quadrant - II (Study Material)

1. Culture & Sensitivity Test

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2. Culture & Sensitivity Test

Additional study material Links 1. Sensitivity - NCDC AMST Link https://ncdc.mohfw.gov.in/wp-content/uploads/2024/03/58495493521681880873.pdf 2. Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health importance in the developing world https://www.who.int/publications/i/item/WHO-CDS-CSR-RMD-2003.6

Quadrant - III (Assignments)

1. Culture & Sensitivity Test

1. Discuss steps involved in culture and identification of organism from a wound of a patient. 2. Describe in detail Kirby-Bauer disc diffusion method of Antimicrobial Susceptibility Testing.

Quadrant - IV (MCQs)

What is the aim of a culture and sensitivity test?

A: To identify pathogens and Screen for effective antibiotics B: To measure antibody levels C: To identify pathogens D: To Screen for effective antibiotics

For collecting a pus sample from a wound for culture, the ideal collection method is:

A: Cotton ball B: Non-sterile gauze C: Sterile swab transported in sterile container D: Saline-soaked paper

What is the recommended incubation temperature for most bacterial cultures?

A: 4°C B: 25°C C: 37°C D: 60°C

What is the recommended incubation temperature for most bacterial cultures?

A: 4°C B: 25°C C: 25°C D: 60°C

What does a positive catalase test result :

A: Agglutination reaction B: Effervesence due to oxygen release C: Precipitation reaction D: No Effervesence formed

The Kirby-Bauer method is used to:

A: Identify fungal species B: Identify fungal species C: Assess antimicrobial susceptibility D: Determine viral load

Which Agar is used for antimicrobial susceptibility testing?

A: Blood agar B: Chocolate agar C: MacConkey agar D: Muller Hinton agar

What does Zone of Inhibition in antimicrobial susceptibility testing suggest

A: Antibiotic is effective against the organism B: Antibiotic is not effective against the organism C: The antibiotic has no effect on the bacteria D: None of above

Which method is commonly used to evenly spread bacteria on agar for antimicrobial susceptibility testing?

A: Streaking with a loop B: Lawn culture using a sterile swab C: Spot inoculation D: Pour plate method

How many discs are used in a plate of 90mm for antimicrobial susceptibility testing ?

A: 4 B: 6 C: 8 D: 10

Which organization provides standard guidelines for antimicrobial susceptibility testing?

A: WHO B: CLSI C: CDC D: ICMR

Validation Certificates

1. Validation certificate

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2. E content module certificate

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3. E content module checking report

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