KVV E-Modules

Quadrant -I (Video)

Cultivation of virus in embryonated egg

Good pasture (1931) was the first who use hen’s embryonated egg for the cultivation of viruses. An embryonated egg provides several sites for the cultivation of viruses. Viz 1. Chorio- …

Quadrant - II (Study Material)

1. Cultivation of virus in embryonated egg

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2. Cultivation of virus in embryonated egg

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3. Cultivation of virus in embryonated egg

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Quadrant - III (Assignments)

1. Cultivation of virus in embryonated egg

Good pasture (1931) was the first who use hen’s embryonated egg for the cultivation of viruses. An embryonated egg provides several sites for the cultivation of viruses. Viz 1. Chorio- allantoic membrane 2. Allantoic cavity 3. Amniotic cavity 4. Yolk sac 5. Embryo

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2. Cultivation of virus in embryonated egg

3. Cultivation of virus in embryonated egg

Quadrant - IV (MCQs)

1. What is the primary advantage of using embryonated eggs for virus cultivation?

A: It is a cost-effective method. B: It provides a natural medium for the virus. C: It allows for the virus to replicate in a living organism. D: It avoids the need for specialized lab equipment.

2. Which part of the embryonated egg is most commonly used for viral inoculation?

A: The egg yolk B: b) The allantoic cavity C: c) The amniotic fluid D: d) The eggshell

What is the typical incubation period for virus growth in an embryonated egg?

A: 12-24 hours B: b) 24-72 hours C: c) 72-96 hours D: d) 96-120 hours

4. Which of the following viruses is commonly propagated using embryonated eggs?

A: HIV B: Influenza virus C: Herpes simplex virus D: Hepatitis C virus

5. What is the main disadvantage of using embryonated eggs for viral cultivation?

A: Limited to viruses that cannot replicate in cell cultures B: Ethical concerns related to the use of animal embryos C: Difficulty in detecting the virus in the egg D: Requires complex genetic modifications to the virus

Which route of administration is used for inoculating a virus into the allantoic cavity of an embryonated egg?

A: Intramuscular injection B: Intravenous injection C: Intra-allantoic injection D: Intra-amniotic injection

7. For the cultivation of avian viruses, which species of egg is commonly used?

A: Chicken eggs B: Duck eggs C: Quail eggs D: Goose eggs

8. Which of the following viruses would typically be inoculated into the amniotic sac of an embryonated egg?

A: Yellow fever virus B: Measles virus C: H5N1 avian influenza virus D: Rabies virus

9. What is a common sign of viral infection in an embryonated egg after inoculation?

A: a) Clear fluid in the egg yolk B: b) Shell discoloration C: c) Embryo death or deformity D: d) Increased hatching rate

10. What is the role of the allantoic fluid in the context of viral cultivation in eggs?

A: a) It serves as a nutrient source for the virus. B: b) It acts as a medium to collect virus after infection. C: c) It protects the embryo from viral infection. D: d) It facilitates the spread of the virus within the egg.

Quadrant -I (Video)

Cultivation of virus in embryonated egg

Quadrant - II (Study Material)

1. Cultivation of virus in embryonated egg

2. Cultivation of virus in embryonated egg

3. Cultivation of virus in embryonated egg

Quadrant - III (Assignments)

1. Cultivation of virus in embryonated egg

2. Cultivation of virus in embryonated egg

3. Cultivation of virus in embryonated egg

Quadrant - IV (MCQs)

1. What is the primary advantage of using embryonated eggs for virus cultivation?

2. Which part of the embryonated egg is most commonly used for viral inoculation?

What is the typical incubation period for virus growth in an embryonated egg?

4. Which of the following viruses is commonly propagated using embryonated eggs?

5. What is the main disadvantage of using embryonated eggs for viral cultivation?

Which route of administration is used for inoculating a virus into the allantoic cavity of an embryonated egg?

7. For the cultivation of avian viruses, which species of egg is commonly used?

8. Which of the following viruses would typically be inoculated into the amniotic sac of an embryonated egg?

9. What is a common sign of viral infection in an embryonated egg after inoculation?

10. What is the role of the allantoic fluid in the context of viral cultivation in eggs?

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