KVV E-Modules - Estimation of Urine Creatinine by Jaffes Method

Quadrant -I (Video)

Quadrant 01: Estimation of Urine Creatinine by Jaffe's Method

Quadrant - II (Study Material)

1. Quadrant 02: Study materials- Part A

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2. Quadrant 02: Study materials- Part B

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3. Quadrant 02: Estimation of Urine Creatinine by Jaffe's Method

Quadrant 02: Estimation of Urine Creatinine by Jaffe's Method

Quadrant - III (Assignments)

1. Quadrant 03: Estimation of Urine Creatinine by Jaffe's Method- Following questions might be asked in the examination

Estimation of Urine Creatinine by Jaffe's Method

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Quadrant - IV (MCQs)

What is the normal reference range of serum creatinine in adult males?

A: 0.2 – 0.6 mg/dL B: 0.6 – 1.2 mg/dL C: 1.5 – 2.5 mg/dL D: 2.5 – 3.5 mg/dL

Which method is commonly used in laboratories for serum creatinine estimation?

A: Jaffe’s method B: Biuret method C: Folin-Wu method D: Benedict’s method

In the Jaffe reaction, creatinine reacts with which chemical to form a colored complex?

A: Sodium hydroxide B: Picric acid C: Sulfuric acid D: Copper sulfate

The color complex formed in the Jaffe’s reaction is:

A: Blue B: Yellow C: Red D: Orange

Which of the following is a major source of error in the Jaffe’s method?

A: Temperature B: Light exposure C: Interference by non-creatinine chromogens D: Hemolysis

What is the significance of elevated serum creatinine levels?

A: Liver dysfunction B: Kidney dysfunction C: Pancreatic inflammation D: Thyroid disease

Why is serum creatinine a good marker for renal function?

A: It is secreted by the liver B: It is reabsorbed by renal tubules C: It is produced at a constant rate and excreted unchanged D: It is stored in muscle tissue

What is the wavelength commonly used for measuring absorbance in the Jaffe’s method using a spectrophotometer?

A: 420 nm B: 490 nm C: 520 nm D: 540 nm

In kinetic Jaffe’s method, why is the reaction rate measured within a specific time interval (e.g., 20–80 seconds)?

A: To maximize the color intensity B: To prevent evaporation of reagents C: To reduce interference from non-creatinine chromogens D: To allow complete reaction of all creatinine

A patient has a serum creatinine level of 2.5 mg/dL. Assuming steady-state creatinine production, what does this most likely indicate?

A: Liver failure B: Acute pancreatitis C: Moderate to severe renal impairment D: Dehydration only

Which of the following compounds may cause false elevation of creatinine in the Jaffe’s reaction, leading to overestimation?

A: Bilirubin B: Ascorbic acid C: Urea D: Sodium chloride

Validation Certificates

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