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KVV E-Modules

Quadrant -I (Video)

Weighing and Measuring Techniques

pH Measurement and Buffer Preparation

API Identification and Purity Tests

Quadrant - II (Study Material)

1. Lab equipments manual

2. Fundamentals of pH

3. Active Pharmaceutical Ingredient (API)

Quadrant - III (Assignments)

1. Assignment on Weighing and Measuring Techniques

Q1. Describe the procedure for performing accurate weighing and volume measurement in a laboratory setting. Highlight the types of equipment used, common sources of error and best practices to ensure precision and accuracy. Instructions: • Support your answer with diagrams where applicable. • Include at least one example for both weighing and volume measurement. • Word Limit: 300–500 words.

2. Assignment on pH Measurement and Buffer Preparation

Q2. Explain the procedure for preparing a buffer solution of known concentration. Determine its pH using appropriate theoretical calculations and experimental methods. Discuss the role of each component in maintaining the pH and the factors affecting buffer capacity. Instructions: • Choose any one buffer system (e.g., Acetic acid/Acetate, Ammonia/Ammonium chloride, etc.). • Show all relevant calculations using the Henderson-Hasselbalch equation. • Mention the equipment and reagents required. • Include a step-by-step experimental procedure for pH measurement. • Word Limit: 400–600 words.

3. Assignment on API Identification and Purity Tests

Q1. Describe the methods used for the identification and purity checking of an Active Pharmaceutical Ingredient (API). Discuss both chemical and instrumental techniques, highlighting the principles, procedures, and significance of each. Instructions: • Mention at least two identification techniques (e.g., IR spectroscopy, melting point determination, TLC). • Describe purity assessment methods (e.g., HPLC, titration, loss on drying, residue on ignition). • Include the role of pharmacopoeial standards (e.g., IP, USP) in API quality control. • Support your answer with examples where appropriate. • Word Limit: 500–700 words.

Quadrant - IV (MCQs)

What is the primary purpose of an analytical balance?

Which of the following is NOT a type of pipette?

How should a burette be properly read?

What is the typical uncertainty in volume measurement when using a 10 mL volumetric pipette?

Why is a volumetric flask used in preparing solutions?

What is the pH range of acidic solutions?

Why is the pH scale important for biological systems?

Which of the following pairs can be used to prepare a buffer solution?

What is the purpose of the Henderson-Hasselbalch equation?

Which of the following is a correct method to prepare a buffer solution?

What is the primary role of an API in a drug formulation?

Regulatory standards for APIs include tests for:

IR spectroscopy is used to identify:

Which of the following chromatography techniques is often used for preliminary identification of an API?

Gas Chromatography is best suited for:

Validation Certificates

1. E content module certificate for weighing & measuring techniques [Validation Part I]

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2. E content module certificate for weighing & measuring techniques [Validation Part II]

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3. E content module Checking report for weighing & measuring techniques

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4. E content module certificate for pH measurement & buffer preparation [Validation Part I]

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5. E content module certificate for pH measurement & buffer preparation [Validation Part II]

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6. E content module Checking report pH measurement & buffer preparation

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7. E content module certificate for API identification & Purity tests [Validation Part I]

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8. E content module certificate for API identification & Purity tests [Validation Part II]

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9. E content module Checking report for API identification & Purity tests

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